Immobilization of Penicillium citrinum by entrapping cells in calcium alginate for the production of neo-fructooligosaccharides

초록

This work describes neo-fiructooligosaccharides (neo-FOSs) production using the immobilized mycelia of Penicillium citrinum. Some critical factors were evaluated to optimize maximal production of neo-FOS. Optimal alginate and cell concentrations were determined to be 1.96% alginate and 7.17% cell, respectively, by statistical analysis. The optimal concentration of CaCl2, which is related to bead stability, was determined to be 2 M. it was possible to increase the neo-FOS production by adding 15 units of glucose oxidase to the batch reaction. By co-iminobilizing cells and glucose oxidase, neo-FOS productivity increased 123% compared with the whole-cell immobilization process. Based on the results above, a coimmobilization technique was developed and it can be utilized for large-scale production.

키워드