Fabrication of protein A LB film for immunoglobulin G immobilization

초록

For the development of preferable immunosensor and protein chip, protein A molecules thin film to have specific affinity to the Fc protion of immunoglobulin G (IgG) was fabricated using Langmuir-Blodgett (LB) technique. The existence of immobilized IgG on the protein A LB film was verified by using fluorescence marker and the fluorescence spectroscopy. The surface morphologies of the protein A LB film and adsorbed IgG layer on the protein A LB film were analyzed by using Atomic Force Microscopy (AFM).

키워드