Toward Artificial Cells: Novel Advances in Energy Conversion and Cellular Motility

초록

The demand to discover every single cellular component has been continuously increasing along with the development of biological techniques. The bottom-up approach to construct a cell-mimicking system from well-defined and tunable compositions is accelerating, with the ultimate goal of comprehending a biological cell. From among the available techniques, the artificial cell has been increasingly recognized as one of the most powerful tools for building a cell-like system from scratch. This review summarizes the development of artificial cells, from a pure giant unilamellar vesicle (GUV) to a controllable, self-fueled proteoliposome, both of which are highly compartmentalized. The basic components of an artificial cell, as well as the optimal conditions required for successful, reproducible GUV formation and protein reconstitution, are discussed. Most importantly, progress in studying the metabolic reactions in and the motility of a reconstituted artificial cell are the main focus of the review. The ability to perform a complicated reaction cascade in a controllable manner is highlighted as a promising perspective to obtaining an autonomous and movable GUV.

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