Three-dimensional tumor model on micro-concave arrayed wells for screening of anticancer drug-loaded nanoparticles

초록

We investigated the effect of anticancer drug-loaded polymeric nanoparticles on three-dimensional breast tumor spheroids. To build MCF7 tumor models, we used concave microwell arrays with 300, 500 and 700 μm in diameter. Doxorubicin-loaded nanoparticles were prepared using thermo-responsive poly(N-isopropylacrylamide) (PNIPAM)-co-acrylic acid (AA). The smallest tumor spheroids cultured in 300 μm microwells were largely disrupted, resulting in a decrease in spheroid diameters upon culturing with doxorubicin-loaded nanoparticles for 96 hours. We found less size difference of largest tumor spheroids and cell viability assay also showed that largest tumor spheroids were highly resistant to the anticancer drug. Therefore, this size-controllable 3D breast tumor model could be a potentially powerful tool for anticancer drug screening applications. © 14CBMS.

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