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Biologically active protein nanoarrays generated using parallel dip-pen nanolithography
- Lee, Seung Woo;
- Oh, Byung-Keun;
- Sanedrin, Raymond G.;
- Salaita, Khalid;
- Fujigaya, Tsuyohiko;
- 외 1명
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135초록
Amine-active N-hydroxysuccinimide-terminated alkyl thiol templates are generated using parallel dip-pen nanolithography (DPN) and are used to covalently couple protein A/G. The protein arrays generated (see figure) are used to capture antibodies through affinity binding, while preserving their biological recognition properties. The versatility of the parallel DPN method for making many similar structures in a relatively high-throughput manner (14 000 dots in 10 min) is described.
키워드
SELF-ASSEMBLED MONOLAYERS; COVALENT ATTACHMENT; SURFACES; NANOFABRICATION; IMMOBILIZATION; MICROARRAYS; ARRAYS; OLIGONUCLEOTIDES; BIOMOLECULES; NANOPLOTTER
- 제목
- Biologically active protein nanoarrays generated using parallel dip-pen nanolithography
- 저자
- Lee, Seung Woo; Oh, Byung-Keun; Sanedrin, Raymond G.; Salaita, Khalid; Fujigaya, Tsuyohiko; Mirkin, Chad A.
- 발행일
- 2006-05-02
- 유형
- Article
- 권
- 18
- 호
- 9
- 페이지
- 1133 ~ +