Quick immunoassay of amyloid beta (Aβ) in exosomes from plasma using magnetic microparticles in water-oil droplets for the diagnosis of Alzheimer's disease

  • Heo, Youhee
  • Park, Minjun
  • Ji, Jae Hoon
  • Shin, Kyeong-Sik
  • Park, Min Cheol
  • ... Shin, Kwan woo
  • 외 1명
Citations

SCOPUS

0

초록

The early diagnosis of Alzheimer's disease (AD) is essential for the effective care of AD patients. Blood-based diagnosis of AD was tackled by measuring oligomer amyloid beta in exosomes. We isolated exosomes from patient plasma using magnetic bead manipulation in aqueous droplets followed by droplet-based ELISA at the same chip. After immunoreaction, the magnetic beads were transported to BEIS sensor to measure the concentration of Aβ in exosome. The simple and quick exosome isolation and ELISA were performed in droplet manipulation chip and discriminating AD patient and normal control was successfully achieved. © 17CBMS-0001.

키워드

Alzheimer's disease (AD)ExosomesMagnetic beadWater-oil droplet
제목
Quick immunoassay of amyloid beta (Aβ) in exosomes from plasma using magnetic microparticles in water-oil droplets for the diagnosis of Alzheimer's disease
저자
Heo, YouheePark, MinjunJi, Jae HoonShin, Kyeong-SikPark, Min CheolShin, Kwan wooKang, Ji Yoon
발행일
2020
유형
Conference Paper
저널명
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
페이지
892 ~ 893