Topical Nanoliposomal Collagen Delivery for Targeted Fibril Formation by Electrical Stimulation

초록

Collagen is a complex, large protein molecule that presents a challenge in delivering it to the skin due to its size and intricate structure. However, conventional collagen delivery methods are either invasive or may affect the protein's structural integrity. This study introduces a novel approach involving the encapsulation of collagen monomers within zwitterionic nanoliposomes, termed Lip-Cols, and the controlled formation of collagen fibrils through electric fields (EF) stimulation. The results reveal the self-assembly process of Lip-Cols through electroporation and a pH gradient change uniquely triggered by EF, leading to the alignment and aggregation of Lip-Cols on the electrode interface. Notably, Lip-Cols exhibit the capability to direct the orientation of collagen fibrils within human dermal fibroblasts. In conjunction with EF, Lip-Cols can deliver collagen into the dermal layer and increase the collagen amount in the skin. The findings provide novel insights into the directed formation of collagen fibrils via electrical stimulation and the potential of Lip-Cols as a non-invasive drug delivery system for anti-aging applications. A novel, non-invasive collagen delivery method using nanoliposomes with electric field stimulation is proposed. Nanoliposomes encapsulating collagen monomers at a low pH (Lip-Cols) penetrate the dermal layer and electric field stimulation facilitates the aggregation of them, leading to the formation of collagen fibril structures. This process can increase the collagen amount in the skin, suggesting potential for effective collagen restoration. image

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