Optimization of Pseudoalteromonas sp JYBCL 1 culture conditions, medium composition and extracellular β-agarase activity

초록

Extracellular agarase produced by the Pseudoalteromonas strain JYBCL 1 is used in a variety of applications in the biotechnology, pharmaceutical, cosmetic, and food industries. The optimization of culture conditions for agarase-producing microbes and agarase activity is thus an important consideration in many industrial applications. In this study, the optimum medium composition and culture conditions for the JYBCL 1 strain were determined using the "one factor at a time" (OFAT) method and a Plackett-Burman design. Optimal cell growth was obtained at a temperature of 25A degrees C and when 10 g/L tryptone was present in the culture medium. Optimal agarase activity occurred at a temperature of 40A degrees C and at pH 6. The presence of carbonyl groups in the extracellular agarase hydrolysis products was verified using FT-IR. LC-MS identified the hydrolyzates as neoagarohexaose, neoagarotetraose, and neoagarobiose. The extracellular agarase produced by the JYBCL 1 strain used in this study was identified as beta-agarase by C-13-NMR spectroscopy.

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