Nano-ELISA using gold nanoparticle-DNA complex for the detection of pathogenic microbe

초록

In this study, we report on the rational design of a nano complex that consists of gold nanoparticles (AuNP), antibody, oligonucleotides and horseradish peroxidase (HRP) for the detection of pathogen existing in contaminated environment. The Digoxigenin(DIG)-labeled oligonucleotides that HRP labeled anti-DIG was bound to DIG to amlify the signal were covalently attached on the AuNP. The interaction forces that resulted from the immune reaction between the functionalized MMP containing target pathogen and AuNPs was used to separate unbound AuNPs. For detection of pathogen, we exploited the reaction of HRP enzyme immobilized on Au probes with tetramethylbenzidine (TMB) that is a substrate of HRP. Using this method, we could detect down to 102 CFU/mL of target pathogen within a hour. This method is very simple and highly sensitive relative to established pathogen biosensors and can be used to detect specific protein markers associated with tumors, bacterial infections or other disease.

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