Development of a universal glycosyltransferase assay amenable to high-throughput formats

Lee, Hyun Soo; Thorson, Jon S.

doi:10.1016/j.ab.2011.06.016

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Development of a universal glycosyltransferase assay amenable to high-throughput formats

Lee, Hyun Soo;
Thorson, Jon S.

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초록

The development of a general 1-Zn(II) nucleoside diphosphate (NDP) sensor assay for rapid evaluation of glycosyltransferase (GT) activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over the corresponding NDP-sugars. Thus, the 1-Zn(II) NDP sensor assay is anticipated to offer broad applicability in the context of GT engineering and characterization. (C) 2011 Elsevier Inc. All rights reserved.

키워드

Glycosyltransferase; Enzyme; Evolution; Engineering; Carbohydrate; Sugar nucleotide; GLYCOSYLATION

제목: Development of a universal glycosyltransferase assay amenable to high-throughput formats

저자: Lee, Hyun Soo; Thorson, Jon S.

DOI: 10.1016/j.ab.2011.06.016

발행일: 2011-11-01

유형: Article

저널명: Analytical Biochemistry

권: 418

호: 1

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