Antibody immobilization for immunosensor on protein a fabricated by electrostatic interaction of synthetic peptide

초록

Protein A thin film was fabricated for antibody immobilization. Cysteine-terminated oligo- peptide composed of polar amino acids was utilized for the promotion of protein A immobilization induced by electrostatic interaction. The formation of the protein A film and the antibody immobilization was investigated with surface plasmon resonance ( SPR) and atomic force microscopy, respectively. As the number of layer deposited on gold increased, the angle of plasmon resonance was increased accordingly. The fabricated antibody thin film was applied to the measurement of bovine serum albumin ( BSA). The measured SPR angle was increased in proportion to the concentration of the applied BSA, of which the detection limit was 100 pM. These experimental results demonstrate that the developed synthetic peptide played an important role in the antibody immobilization for immunosensors.

키워드