Free radical initiated peptide sequencing using MALDI-TOF/TOF mass spectrometry

초록

In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [a<inf>n</inf>+H]+, [z<inf>n</inf>+H]+, and [y<inf>n</inf>]+-type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS. © Korean Society for Mass Spectrometry.

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