Single-Molecule DNA Visualization

초록

Direct visualization of individual DNA molecules is a powerful platform for understanding and managing genomic and biochemical phenomena in the context of DNA sequences. Although sequencing technology at the single nucleotide level has advanced dramatically, there are still numerous unsolved biological problems that are limited by DNA fragmentation during the sequencing procedure. The ultimate goal of DNA analysis is to search for a target gene by its sequence and to control its expression on chromosomal DNA without fragmentation or amplification. Given these concerns, single-molecule DNAvisualization is the first step in identifying and controlling target genes of large DNA molecules. Advancements in this field include developments in single-molecule methods, advanced microscopy techniques, molecular labeling modalities, and nano-/microfluidics. This chapter describes these developments in terms of single-molecule DNA visualization. © Springer Nature Singapore Pte Ltd. 2023.

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