Development of Triglyceride Certified Reference Materials in Human Frozen Serum Using Isotope Dilution-Liquid Chromatography-Tandem Mass Spectrometry

초록

Certified reference materials (CRMs), KRISS CRM 111-01-010 and 111-01-011, were first developed in Korea to provide SI-traceability in the measurement of triglycerides (TGs) in human frozen serum. For absolute TG quantification in KRISS CRMs, isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) was used. Both certification and short-term stability assessment of TG in KRISS CRMs were performed for each of 10 and eight selected units. The TG certified values and expanded uncertainties in KRISS CRMs were as follows: 0.608 +/- 0.025 mmol/kg for CRM 111-01-010 and 1.825 +/- 0.072 mmol/kg for CRM 111-01-011. The short-term TG stability results were not significantly changed relative to the certified values, and therefore both KRISS CRMs were stable during the monitoring period at each temperature. Based on our experiments, the developed KRISS CRMs can provide accurate, precise, and reproducible TG measurements in human frozen serum for clinical laboratories with SI-traceability.

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